We supply an integrated biochemistry training lab system that covers techniques used in the biochemical experiments such as principles and practices of Biochemistry, chromatography for the investigation of metabolites, polysaccharides, application of enzymes for quantitative determinations by spectroscopic methods, and introductory experiments about the kinetics of enzyme action and basic biochemical principles of proteins.
The lab equipment package enables students to apply many experiments and activities cover various topics in Biochemistry such as:
- Laboratory Calculations and Instrumentation
- pH Measurement and Buffer Preparation
- Titration of unknown Amino Acid
- Chromatographic Analysis of Amino Acids
- Spectrophotometers and Protein Determination
- Enzyme Kinetics
- Protein Purification and Analysis on SDS-PAGE
- Qualitative Test for Carbohydrates
- Glycolysis and Fermentation in Yeast
- Ionic permeability of the cell membrane
- Determination of the Michaelis constant
- Enzymatic activity of catalase
- Substrate inhibition of enzymes
- Enzyme inhibition (poisoning of enzymes)
- Determination of the isoelectric point of various amino acids
- Fermentation of molasse to ethanol
- Protein Purification
- Salting Out
- Dialysis
- Gel Filtration
- Expression and Characterization of a Recombinant Enzyme in E.coli.
- SDS Gel Electrophoresis, Molecular Weight Measurements; Protein Gel Staining (including the whole-cell homogenates); Gel Drying
- Western Blotting Concepts; SDS PAGE and Transfer
- Western Blot Analysis
